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Correlation of follicular fluid hormones with embryonic development. a Schematic representation of patient selection, sample inclusion, fertilisation and embryo development. SET: Single Embryo Transfer; DET: Double Embryo Transfer; PN: Pronucleus; +hCG: positive human chorionic gonadotropin (pregnancy) test. b Diagrammatic representation of differential oocyte quality and subsequent embryonic developmental competence. Follicular fluid used in the current study was isolated from the first follicular aspiration, during oocyte recovery at a private fertility clinic. The developmental outcome of each oocyte was recorded based on whether they reached blastocyst on Day 5 of in vitro embryo culture. Samples were classified as either fertilising and developing to blastocyst (BLAST) or fertilising but failing to develop to blastocyst (FERT). Corresponding follicular fluid was evaluated for anti-Mullerian hormone (AMH) and progesterone (P4) protein levels. This figure was prepared using the Biomedical PPT toolkit suite (www.

No funding or sponsorship was received for this study. The authors would like to thank the Society of Clinical Investigation for its support of this project through its annual research day. We are very grateful to Dr Susan Crum for providing advice regarding data analysis of the data. The authors would also like to thank Mr Daniel Dolan for his assistance in data collection. We would also like to thank the two anonymous reviewers for their helpful suggestions and comments on an earlier version of this manuscript.

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